Rabbit anti‑Human CASP3 / Caspase 3 IgG Polyclonal RW-C12494-50
Antibody: CASP3 / Caspase 3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody, Application: WB, Reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit, Sheep, Chicken, Yeast, Format: Unconjugated, Unmodified, Descriptio: Caspase 3 antibody RW-C12494 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for WB., Targe: Human CASP3 / Caspase 3, Synonym: CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit, Sheep, Chicken, Yeast (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Unconjugated, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: Recombinant full length human caspase-3 protein with an N-Terminus (His) 6 tag., Epitop: N-Terminus, Specificit: Recognizes human caspase-3. In apoptotic cells, this antibody also detects 20kD and 18kD proteins which correspond to the processed forms of caspase-3. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis. A weak ~30kD band may be detected in some samples. This band is not caspase-3 as it is detected in MCF7 cell lysates which lack the caspase-3 protein. Species cross-reactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit and yeast., Application: Western blot (1 µg/ml), Usag: Suitable for use in Western Blot. Western Blot (ECL): 1 ug/ml. A band is seen at ~36kD. Positive control: HeLa Nuclear Extract., Presentatio: PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CASP3 / Caspase : Uniprot: P42574 NCBI: NM_004346 NP_004337.2