Rabbit anti‑Human CASP3 / Caspase 3 Polyclonal RW-B3404-50
Antibody: CASP3 / Caspase 3 Rabbit anti-Human Polyclonal Antibody, Application: IHC, IHC-P, WB, Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Rabbit, Chicken, Yeast, Format: Unconjugated, Unmodified, Descriptio: CASP3 (Caspase 3) is a cysteine-aspartic acid protease involved in the execution-phase of cell apoptosis. In axonal injury, CASP3 mediates the cleavage of amyloid-beta 4A precursor protein which results in amyloid beta peptide deposition. Activation of CASP3 is correlated with neuronal death and functional decline in Alzheimer's disease. CASP3 is also vital for the development of the normal brain, and is involved in embryonic and hematopoietic stem cell differentiation. Alternative splicing of this gene results in two transcript variants that encode the same protein. In immunohistochemistry, CASP3 has high cytoplasmic positivity in lymphoid tissues, and is found in most other tissues throughout the body.
References: J Neurotrauma. 2002 May;19(5):601-14, PMID: 12042095; Brain Res. 2001 Apr 20;898(2):350-7, PMID: 11306022; Cell. 1999 Apr 30;97(3):395-406, PMID: 10319819; Cell Stem Cell. 2 (6): 515–6, PMID: 18522841;, Targe: Human CASP3 / Caspase 3, Synonym: CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama, Hos: Rabbit, Reactivit: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Rabbit, Chicken, Yeast (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: Recombinant human Caspase-3 protein (full length)., Application: IHC
IHC - Paraffin (10 µg/ml)
Western blot (1:1000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Immunohistochemistry: RW-B3404 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for RW-B3404 was determined to be 10 ug/ml., Presentatio: PBS, 0.09% Sodium Azide, 50% Glycerol, Storag: Store at -20°C. Aliquot to avoid freeze/thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CASP3 / Caspase : Uniprot: P42574 NCBI: NM_004346 NP_004337.2